Neuroscience

Last Updated on Saturday, 18 February 2012 21:38

Applications of High Content in Neuroscience

In the area of neurobiology, cellular morphology is very important. Screening outputs such as neurite outgrowth, branching can only be detected with an imaging approach and other targets such as synaptogenesis benefit from being co-localised with the neurite. Thermo Fisher Scientific scientists and our customers have published scientific data using the Neurite Outgrowth, Extended Neurite Outgrowth and Neuronal Profling BioApplications and, thus validating the high content approach to neuronal morphological analysis. In many cases samples can be prepared with the Thermo Scientific high content reagents, which providea a validated set of reagents and sample preparation protocol to ensure success in these assay types.

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Delivering the tools for high-content neuronal research

Journal Citations by Thermo Scientific users

1. McIlvain HB, A Baudy, K Sullivan, D Liu, K Pong, M Fennell, J Dunlop; Wyeth Research (2006) Pituitary adenylate cyclase-activating peptide (PACAP) induces differentiation in the neuronal F11 cell line through a PKA-dependent pathway Brain Res 1077(1):16-23. PubMed Abstract
2. Fennell M, H Chan, A Wood; Wyeth Research (2006). Multiparameter Measurement of Caspase 3 Activation and Apoptotic Cell Death in NT2 Neuronal Precursor Cells Using High-Content Analysis. J Biomol Screen 11(3):296-302 Abstract at Publisher Site
3. Resnick L, M Fennell; Wyeth-Ayerst Research (2004). Targeting JNK3 for the treatment of neurodegenerative disorders. Drug Discov Today 9(21): 932-9. PubMed Abstract
4. Richards GR, RM Millard, M Leveridge, J Kerby, PB Simpson; Merck Sharp & Dohme (2004). Quantitative Assays of Chemotaxis and Chemokinesis for Human Neural Cells. Assay Drug Dev Tech 2: 465-472. Publisher Link
5. Schlag BD, Z. Lou, M. Fennell, J. Dunlop; Wyeth-Ayerst Research (2004). Ligand dependency of 5-HT2C receptor internalization. J Pharmacol Exp Ther 310: 865-870. PubMed Link
6. Li Z, Y Yan, EA Powers, X Ying, K Janjua, T Garyantes, B Baron; Aventis (2003) Identification of gap junction blockers using automated fluorescence microscopy imaging. J Biomol Screening 8: 489-499. PubMed Abstract
7. Simpson PB, JI Bacha, EL Palfreyman, AJ Woollacott, RM McKernan, J Kerby; Merck Sharp & Dohme (2001) Retinoic acid-evoked differentiation of neuroblastoma cells predominates over growth factor stimulation: an automated image capture and quantitation approach to neuritogenesis. Anal Biochem 298: 163-169 Abstract

Scientific Posters – Posters are available for download from our HCSLibrary

1. Cuda M, J Callio, C Chu, L Reuter, E Ramer, JR Haskins; Univ. Pittsburgh School of Medicine and Cellomics, Inc. (2005). Extending High Content Analysis to Models of Animal Injury. Presented at the 11^th Annual Meeting of the Society for Biomolecular Screening.
2. Stewart W, M Fennell, M Lai, M Pausch, P Patel and J Dunlop; Wyeth Research (2004). A Cell Based, High Content Screening Assay for Visualization of Neurotensin I Receptor Internalization Using the Cellomics Arrayscan. Presented at the 10^th Annual Meeting of the Society for Biomolecular Screening.
3. DeBiasio R and RN Ghosh (2004). The Use of Cryopreserved Primary Neurons in High Content Screening Assays. Presented at the 10^th Annual Meeting of the Society for Biomolecular Screening.
4. Abraham VC, RN Ghosh, JR Haskins, P Petrosko, and P Sammak; Magee-Women's Research Institute and Cellomics, Inc. (2004). Application of High Content Screening to the Analysis of Stem Cell Growth and Differentiation. Presented at the 10^th Annual Meeting of the Society for Biomolecular Screening.
5. McIlvain HB, R Mark, S Campos, A Baudy, D Liu, K Pong, A Wood, J Dunlop, M Fennell; Wyeth Research (2004) Modulation of neurite outgrowth in cultured neurons as measured by high content screening methods. Presented at the Annual Meeting of the Society for Neuroscience. Reprint available upon request.
6. Ramer E, S Arden, R Ghosh and JR Haskins (2003). Automated Cell-Based Quantitation of Neurite Outgrowth and Neuronal Cell Differentiation Using Cellomics’ Neurite Outgrowth BioApplications. Presented at the 9^th Annual Meeting of the Society for Biomolecular Screening.

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